University of Wisconsin–Madison

Deane F. Mosher

Professor (also Medicine)

(608) 262-1576

5428A Biochemical Sciences Building
440 Henry Mall, Madison, WI 53706

The Mosher Lab Website


• M.D. 1968, Harvard Medical School
• Postdoctoral 1970-72, Harvard Medical School (Elkan Blout)

Honors & Awards

• Fellow, American Association for the Advancement of Science, 2015
• Mentoring in the Basic Sciences, American Society of Hematology, 2006
• M.D. (Honorary), Lund University, Sweden, 1998
• R.F. Schilling WARF Professor, 1997
• H.I. Romnes Fellow,1982-87
• Established Investigator, American Heart Association and its Wisconsin Affiliate, 1978-83

Research Interests

My laboratory has been a leader in characterization of structure and function of molecules of blood plasma, platelets, and extracellular matrix. We are best known for studies of fibronectin, thrombospondins, and vitronectin and participate in collaborative studies that utilize the reagents and expertise that we have developed over the years..

For the past two decades, we have worked closely with colleagues in Allergy and Pulmonary and Critical Care Medicine to study activation and trafficking of eosinophils in asthma. Current asthma-related projects include characterizing the proteomes and phosphoproteomes of resting and activated eosinophils (in collaboration with the Josh Coon laboratory); describing the molecular anatomy of and signaling behind the radical changes in eosinophils that occur upon activation with cytokines, chemokines, and other mediators; exploring effects of interactions with periostin, an extracellular matrix protein deposited in the asthmatic lung, on behavior of eosinophils; and identification of biomarkers that differentiate among different types and stages of asthma. In addition, we study adhesion to extracellular matrix proteins of bacteria that are respiratory pathogens or commensal to the bronchial tree.

Recent Publications

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• Ma W, Ma H, Fogerty FJ, Mosher DF (2015) Bivalent Ligation of the Collagen-binding Modules of Fibronectin by SFS, a Non-anchored Bacterial Protein of Streptococcus equi. J. Biol. Chem. 290(8):4866-76 (PMC4335226)

• Raval KK, Tao R, White BE, De Lange WJ, Koonce CH, Yu J, Kishnani PS, Thomson JA, Mosher DF, Ralphe JC, Kamp TJ (2015) Pompe Disease results in a Golgi-based Glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes. J. Biol. Chem. 290(5):3121-36 (PMC4317045)

• Wang Y, Reheman A, Spring CM, Kalantari J, Marshall AH, Wolberg AS, Gross PL, Weitz JI, Rand ML, Mosher DF, Freedman J, Ni H (2014) Plasma fibronectin supports hemostasis and regulates thrombosis. J. Clin. Invest. 124(10):4281-93 (PMC4191008)

• Harris G, Ma W, Maurer LM, Potts JR, Mosher DF (2014) Borrelia burgdorferi protein BBK32 binds to soluble fibronectin via the N-terminal 70-kDa region, causing fibronectin to undergo conformational extension. J. Biol. Chem. 289(32):22490-9 (PMC4139255)

• Mosher DF (2014) Factor XIII and adipocyte biology. Blood 124(8):1213-4 (PMC4141509)

• Han ST, Mosher DF (2014) IL-5 induces suspended eosinophils to undergo unique global reorganization associated with priming. Am. J. Respir. Cell Mol. Biol. 50(3):654-64 (PMC4068932)

• Esnault S, Kelly EA, Johansson MW, Liu LY, Han ST, Akhtar M, Sandbo N, Mosher DF, Denlinger LC, Mathur SK, Malter JS, Jarjour NN (2014) Semaphorin 7A is expressed on airway eosinophils and upregulated by IL-5 family cytokines. Clin. Immunol. 150(1):90-100 (PMC3947215)

• Sabatier L, Djokic J, Fagotto-Kaufmann C, Chen M, Annis DS, Mosher DF, Reinhardt DP (2013) Complex contributions of fibronectin to initiation and maturation of microfibrils. Biochem. J. 456(2):283-95

• Beene LC, Wang LW, Hubmacher D, Keene DR, Reinhardt DP, Annis DS, Mosher DF, Mecham RP, Traboulsi EI, Apte SS (2013) Nonselective assembly of fibrillin 1 and fibrillin 2 in the rodent ocular zonule and in cultured cells: implications for Marfan syndrome. Invest. Ophthalmol. Vis. Sci. 54(13):8337-44 (PMC3875392)

• Johansson MW, Kruger SJ, Schiebler ML, Evans MD, Sorkness RL, Denlinger LC, Busse WW, Jarjour NN, Montgomery RR, Mosher DF, Fain SB (2013) Markers of vascular perturbation correlate with airway structural change in asthma. Am. J. Respir. Crit. Care Med. 188(2):167-78 (PMC3778758)

• Moussavi-Harami SF, Annis DS, Ma W, Berry SM, Coughlin EE, Strotman LN, Maurer LM, Westphall MS, Coon JJ, Mosher DF, Beebe DJ (2013) Characterization of molecules binding to the 70K N-terminal region of fibronectin by IFAST purification coupled with mass spectrometry. J. Proteome Res. 12(7):3393-404 (PMC3832424)

• Johansson MW, Annis DS, Mosher DF (2013) α(M)β(2) integrin-mediated adhesion and motility of IL-5-stimulated eosinophils on periostin. Am. J. Respir. Cell Mol. Biol. 48(4):503-10 (PMC3653603)

• Johansson MW, Mosher DF (2013) Integrin activation States and eosinophil recruitment in asthma. Front Pharmacol 4:33 (PMC3612688)

• Johansson MW, Gunderson KA, Kelly EA, Denlinger LC, Jarjour NN, Mosher DF (2013) Anti-IL-5 attenuates activation and surface density of β(2) -integrins on circulating eosinophils after segmental antigen challenge. Clin. Exp. Allergy 43(3):292-303 (PMC3579563)

• Shelef MA, Bennin DA, Mosher DF, Huttenlocher A (2012) Citrullination of fibronectin modulates synovial fibroblast behavior. Arthritis Res. Ther. 14(6):R240 (PMC3674601)

• Tomasini-Johansson BR, Johnson IA, Hoffmann FM, Mosher DF (2012) Quantitative microtiter fibronectin fibrillogenesis assay: use in high throughput screening for identification of inhibitor compounds. Matrix Biol. 31(6):360-7 (PMC3508085)

• Tsang TM, Annis DS, Kronshage M, Fenno JT, Usselman LD, Mosher DF, Krukonis ES (2012) Ail protein binds ninth type III fibronectin repeat (9FNIII) within central 120-kDa region of fibronectin to facilitate cell binding by Yersinia pestis. J. Biol. Chem. 287(20):16759-67 (PMC3351329)

• Maurer LM, Ma W, Eickstaedt NL, Johnson IA, Tomasini-Johansson BR, Annis DS, Mosher DF (2012) Ligation of the fibrin-binding domain by β-strand addition is sufficient for expansion of soluble fibronectin. J. Biol. Chem. 287(16):13303-12 (PMC3339936)

• Mosher DF, Adams JC (2012) Adhesion-modulating/matricellular ECM protein families: a structural, functional and evolutionary appraisal. Matrix Biol. 31(3):155-61 View Abstract · Pubmed Record

• Hoffmann BR, Liu Y, Mosher DF (2012) Modification of EGF-like module 1 of thrombospondin-1, an animal extracellular protein, by O-linked N-acetylglucosamine. PLoS ONE 7(3):e32762 (PMC3293841)