University of Wisconsin–Madison

Deane F. Mosher

Professor (also Medicine)

dfm1@medicine.wisc.edu

(608) 262-1576

5428A Biochemical Sciences Building
440 Henry Mall, Madison, WI 53706

The Mosher Lab Website

Education

• M.D. 1968, Harvard Medical School
• Postdoctoral 1970-72, Harvard Medical School (Elkan Blout)

Honors & Awards

• Fellow, American Association for the Advancement of Science, 2015
• Mentoring in the Basic Sciences, American Society of Hematology, 2006
• M.D. (Honorary), Lund University, Sweden, 1998
• R.F. Schilling WARF Professor, 1997
• H.I. Romnes Fellow,1982-87
• Established Investigator, American Heart Association and its Wisconsin Affiliate, 1978-83

Research Interests

My laboratory has been a leader in characterization of structure and function of molecules of blood plasma, platelets, and extracellular matrix. We are best known for studies of fibronectin, thrombospondins, and vitronectin and participate in collaborative studies that utilize the reagents and expertise that we have developed over the years..

For the past two decades, we have worked closely with colleagues in Allergy and Pulmonary and Critical Care Medicine to study activation and trafficking of eosinophils in asthma. Current asthma-related projects include characterizing the proteomes and phosphoproteomes of resting and activated eosinophils (in collaboration with the Josh Coon laboratory); describing the molecular anatomy of and signaling behind the radical changes in eosinophils that occur upon activation with cytokines, chemokines, and other mediators; exploring effects of interactions with periostin, an extracellular matrix protein deposited in the asthmatic lung, on behavior of eosinophils; and identification of biomarkers that differentiate among different types and stages of asthma. In addition, we study adhesion to extracellular matrix proteins of bacteria that are respiratory pathogens or commensal to the bronchial tree.

Recent Publications

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• Bortnov V, Annis DS, Fogerty FJ, Barretto KT, Turton KB, Mosher DF (2018) Myeloid-derived growth factor is a resident endoplasmic reticulum protein. J. Biol. Chem. 293(34):13166-13175.

• Kumra H, Sabatier L, Hassan A, Sakai T, Mosher DF, Brinckmann J, Reinhardt DP (2018) Roles of fibronectin isoforms in neonatal vascular development and matrix integrity. PLoS Biol. 16(7):e2004812 (PMC6072322)

• Schnellmann R, Sack R, Hess D, Annis DS, Mosher DF, Apte SS, Chiquet-Ehrismann R (2018) A Selective Extracellular Matrix Proteomics Approach Identifies Fibronectin Proteolysis by A Disintegrin-like and Metalloprotease Domain with Thrombospondin Type 1 Motifs (ADAMTS16) and Its Impact on Spheroid Morphogenesis. Mol. Cell Proteomics 17(7):1410-1425 (PMC6030725)

• Turton KB, Wilkerson EM, Hebert AS, Fogerty FJ, Schira HM, Botros FE, Coon JJ, Mosher DF (2018) Expression of novel “LOCGEF” isoforms of ARHGEF18 in eosinophils. J. Leukoc. Biol. 104(1):135-145 (PMC6013370)

• Esnault S, Hebert AS, Jarjour NN, Coon JJ, Mosher DF (2018) Proteomic and Phosphoproteomic Changes Induced by Prolonged Activation of Human Eosinophils with IL-3. J. Proteome Res. 17(6):2102-2111 (PMC5984179)

• Kelly EA, Esnault S, Liu LY, Evans MD, Johansson MW, Mathur S, Mosher DF, Denlinger LC, Jarjour NN (2017) Mepolizumab Attenuates Airway Eosinophil Numbers, but Not Their Functional Phenotype, in Asthma. Am. J. Respir. Crit. Care Med. 196(11):1385-1395 (PMC5736971)

• Johansson MW, Khanna M, Bortnov V, Annis DS, Nguyen CL, Mosher DF (2017) IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration. Clin. Exp. Allergy 47(10):1263-1274 (PMC5623171)

• Esnault S, Johansson MW, Kelly EA, Koenderman L, Mosher DF, Jarjour NN (2017) IL-3 up-regulates and activates human eosinophil CD32 and αMβ2 integrin causing degranulation. Clin. Exp. Allergy 47(4):488-498 (PMC5378663)

• Turton KB, Esnault S, Delain LP, Mosher DF (2016) Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts. J Vis Exp (116): (PMC5092172)

• Wilkerson EM, Johansson MW, Hebert AS, Westphall MS, Mathur SK, Jarjour NN, Schwantes EA, Mosher DF, Coon JJ (2016) The Peripheral Blood Eosinophil Proteome. J. Proteome Res. 15(5):1524-33 (PMC5222579)

• Nett JE, Cabezas-Olcoz J, Marchillo K, Mosher DF, Andes DR (2016) Targeting Fibronectin To Disrupt In Vivo Candida albicans Biofilms. Antimicrob. Agents Chemother. 60(5):3152-5 (PMC4862492)

• Johansson MW, Annis DS, Mosher DF (2013) α(M)β(2) integrin-mediated adhesion and motility of IL-5-stimulated eosinophils on periostin. Am. J. Respir. Cell Mol. Biol. 48(4):503-10 (PMC3653603)

• Johansson MW, Mosher DF (2013) Integrin activation States and eosinophil recruitment in asthma. Front Pharmacol 4:33 (PMC3612688)

• Zheng M, Turton KB, Zhu F, Li Y, Grindle KM, Annis DS, Lu L, Drennan AC, Tweardy DJ, Bharadwaj U, Mosher DF, Rui L (2016) A mix of S and ΔS variants of STAT3 enable survival of activated B-cell-like diffuse large B-cell lymphoma cells in culture. Oncogenesis 4:e184 (PMC4728674)

• Nett JE, Zarnowski R, Cabezas-Olcoz J, Brooks EG, Bernhardt J, Marchillo K, Mosher DF, Andes DR (2015) Host contributions to construction of three device-associated Candida albicans biofilms. Infect. Immun. 83(12):4630-8 (PMC4645408)

Mosher DF, Johansson MW, Gillis ME, Annis DS (2015) Periostin and TGF-β-induced protein: Two peas in a pod? Crit. Rev. Biochem. Mol. Biol. 50(5):427-39 (PMC4606919)

• Annis DS, Ma H, Balas DM, Kumfer KT, Sandbo N, Potts GK, Coon JJ, Mosher DF (2015) Absence of Vitamin K-Dependent γ-Carboxylation in Human Periostin Extracted from Fibrotic Lung or Secreted from a Cell Line Engineered to Optimize γ-Carboxylation. PLoS ONE 10(8):e0135374 (PMC4537219)

• Liu Z, Morgan S, Ren J, Wang Q, Annis DS, Mosher DF, Zhang J, Sorenson CM, Sheibani N, Liu B (2015) Thrombospondin-1 (TSP1) contributes to the development of vascular inflammation by regulating monocytic cell motility in mouse models of abdominal aortic aneurysm. Circ. Res. 117(2):129-41 (PMC4490953)

• Turton KB, Annis DS, Rui L, Esnault S, Mosher DF (2015) Ratios of Four STAT3 Splice Variants in Human Eosinophils and Diffuse Large B Cell Lymphoma Cells. PLoS ONE 10(5):e0127243 (PMC4436176)

• Ma W, Ma H, Mosher DF (2015) On-Off Kinetics of Engagement of FNI Modules of Soluble Fibronectin by β-Strand Addition. PLoS ONE 10(4):e0124941 (PMC4412574)