Jon Audhya
Credentials: Regulation of membrane transport during development and disease
Position title: Professor and Assoc. Dean for Basic Research
Email: audhya@wisc.edu
Phone: (608) 262-3761
Address:
5214 Biochemical Sciences Building
440 Henry Mall, Madison, WI 53706
The Audhya Lab Website
Education
• B.S. 1997, Brown University, Providence, RI
• Ph.D. 2002, University of California, San Diego (S. Emr)
• Postdoctoral 2003-07, Ludwig Institute for Cancer Research (K. Oegema)
Honors & Awards
• Sigma Xi Honorary Society, 1997
• Bachelor of Science, Magna cum Laude, Brown University, 1997
• Harvey Almy Baker Graduate Fellowship, Brown University, 1997
• National Cancer Institute Training Grant Award, 1998-2002
• Helen Hay Whitney Foundation Post-Doctoral Fellowship, 2004-2007
• DeLill Nasser Award for Professional Development in Genetics, 2006
• March of Dimes Basil O’Connor Starter Scholar Research Award, 2010
• American Heart Association Scientist Development Grant Award, 2010
• Shaw Scientist Award, 2010
• American Cancer Society Research Scholar Award, 2012
• Vilas Associate Award, University of Wisconsin-Madison, 2015
• Vilas Faculty Early Career Investigator Award, University of Wisconsin-Madison, 2016
• H.I. Romnes Faculty Fellowship, UW Graduate School/WARF, 2017
• Tom Wahlig Foundation Advanced Scholarship, 2018
Research Interests
Our laboratory is committed to understanding fundamental mechanisms by which membrane proteins, lipids, and other macromolecules are transported throughout eukaryotic cells. To do so, we take advantage of numerous interdisciplinary approaches, including biochemistry, structural biology, biophysics, genetics, molecular biology and high resolution fluorescence and electron microscopy.
Additionally, we use a variety of experimental systems, ranging from simple animal models (e.g. Caenorhabditis elegans) to human induced pluripotent stem cells (iPSCs). We also aim to recapitulate individual steps of membrane transport in vitro, using recombinant proteins and chemically defined lipids. Our ultimate goal is to identify the regulatory pathways that control membrane deformation, which enable vesicle formation in the endosomal and secretory systems. Although basic research is the cornerstone of our program, we also seek to define pathomechanisms that underlie human disease, focusing on the impact of mutations in key trafficking components that lead to cancer, neurodegeneration, asthma, and diabetes. For more information, please visit our lab webpage: www.audhyalab.org .
Publications
Perform a customized PubMed literature search for Jon Audhya
- Zhang, F., M. Zhao, D.R. Braun, S.S. Ericksen, J.S. Piotrowski, J. Nelson, J. Peng, G.E. Ananiev, S. Chanana, K. Barns, J. Fossen, H. Sanchez, M.G. Chevrette, I.A. Guzei, C. Zhao, L. Guo, W. Tang, C.R. Currie, S.R. Rajski, A. Audhya, D.R. Andes, and T.S. Bugni. (2020). A marine microbiome antifungal targets urgent-threat drug-resistant fungi. Science (New York, N.Y.), 370: 974-978.
- Penfield, L., R. Shankar, E. Szentgyörgyi, A. Laffitte, M.S. Mauro, A. Audhya, T. Müller-Reichert, and S. Bahmanyar. (2020). Regulated lipid synthesis and LEM2/CHMP7 jointly control nuclear envelope closure. The Journal of cell biology, 219: .
- Mandal, T., W. Lough, S.E. Spagnolie, A. Audhya, and Q. Cui. (2020). Molecular Simulation of Mechanical Properties and Membrane Activities of the ESCRT-III Complexes. Biophysical journal, 118: 1333-1343.
- Peotter, J., W. Kasberg, I. Pustova, and A. Audhya. (2019). COPII-mediated trafficking at the ER/ERGIC interface. Traffic (Copenhagen, Denmark), 20: 491-503.
- Wan, J., S. Block, C.M. Scribano, R. Thiry, K. Esbona, A. Audhya, and B.A. Weaver. (2019). Mad1 destabilizes p53 by preventing PML from sequestering MDM2. Nature communications, 10: 1540.
- Quinney, K.B., E.B. Frankel, R. Shankar, W. Kasberg, P. Luong, and A. Audhya. (2019). Growth factor stimulation promotes multivesicular endosome biogenesis by prolonging recruitment of the late-acting ESCRT machinery. Proceedings of the National Academy of Sciences of the United States of America, 116: 6858-6867.
- Block, S., A.L. Schuh, and A. Audhya. (2019). Biochemical Approaches to Studying Caenorhabditis elegans ESCRT Functions In Vitro. Methods in molecular biology (Clifton, N.J.), 1998: 189-202.
- Jones, J.R., L. Kong, M.G. Hanna, B. Hoffman, R. Krencik, R. Bradley, T. Hagemann, J. Choi, M. Doers, M. Dubovis, M.A. Sherafat, A. Bhattacharyya, C. Kendziorski, A. Audhya, A. Messing, and S.C. Zhang. (2018). Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes. Cell reports, 25: 947-958.e4.
- Schenk, N.A., P.J. Dahl, M.G. Hanna, A. Audhya, G.G. Tall, J.D. Knight, and A. Anantharam. (2018). A simple supported tubulated bilayer system for evaluating protein-mediated membrane remodeling. Chemistry and physics of lipids, 215: 18-28.
- Slosarek, E.L., A.L. Schuh, I. Pustova, A. Johnson, J. Bird, M. Johnson, E.B. Frankel, N. Bhattacharya, M.G. Hanna, J.E. Burke, D.A. Ruhl, K. Quinney, S. Block, J.L. Peotter, E.R. Chapman, M.D. Sheets, S.E. Butcher, S.M. Stagg, and A. Audhya. (2018). Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation. Cell reports, 24: 2248-2260.