Andrea A. Putnam

The Putnam Lab Website

2008 T32 Predoctoral Institutional National Research Service Award
2016 Doctoral Excellence Award in Biochemistry
2019 F32 Ruth L. Kirschstein Postdoctoral Individual National Research Service Award

Research Interests

Function and regulation of RNA condensates in development

Cells are organized into membrane-bound and membraneless structures referred to as condensates. Condensates contain concentrated biomolecules, including protein and nucleic acid, and are suggested to play a role in nearly all biological processes. However, in most cases, the function of condensates has not been shown experimentally. Challenges assigning functions arise in part because condensates are a complex mix of many biomolecules, including proteins and nucleic acids. Additionally, condensates are dynamic and sensitive to environmental changes, including cell cycle, post-translation modification, and stress. The focus of my lab is to understand the molecular details of condensates using live cell imaging of endogenous condensates and in vitro reconstitution.

Approach: We couple observations in live cells with genetic, biochemical, biophysical, and theoretical analysis. We use techniques including super-resolution microscopy, quantitative live cell imaging, biophysical measurements, single molecule imaging, and modeling to understand condensate assembly at the molecular level.

Germ granules: Germ granules are large condensates ribonucleoprotein (RNP) organelles found in the germ cells, embryos, oocytes, and sperm of most, if not all, animals. Germ granules are suggested to have a wide range of functions important for germline development and inheritance, and when granules are compromised, organisms exhibit germline defects. We use Caenorhabditis elegans as a powerful model for studying germ granule condensates. C. elegans is amenable to live cell super-resolution imaging and analysis in an intact animal and has an extensive genetics tool box including CRISPR and RNAi.

Condensate substructure: Many cellular condensates are not homogenous but instead have substructure. In C. elegans, we found that a type of germ granule, P granules, is composed of a dynamic liquid-like core surrounded by less dynamic solid-like clusters. The solid clusters adsorb to the surface of the liquid layer, reduce surface tension, and prevent coarsening. This phenomenon is known in material science as Pickering stabilization and was first described over a century ago. Using techniques developed to study P granules, we will explore how condensate substructure and material properties impacts function and regulation.

Condensation and enzymatic activity:  Condensates contain enzymes that modify proteins, small molecules, and nucleic acids. In artificial and in vitro reconstituted systems, condensates can both enhance and inhibit enzyme activity. Disruption of enzymes can also alter the formation, morphology, and material properties of condensates in cells. Using enzymes enriched germ granules, we will examine how condensation regulates enzymatic activity and, conversely, how activity can regulate condensation.

Publications

1. Putnam A, Thomas L, Seydoux G. (2023) RNA granules: functional compartments or accidental condensates. Genes and Development 37:354–376  Link
2. Thomas L, Putnam A, Folkmann A. (2023) Germ granules in development. Development. 150(2). Link
3. Putnam A, Seydoux G. Intrinsically disordered regions: a platform for regulated assembly of biomolecular condensates. In: Uversky VN, editor. Droplets of Life. 1 ed. Cambridge, MA: Academic Press; 2022. Chapter 13; p.397-430. 728p. Link
4. Folkmann, A.*, Putnam, A.*, Lee, C.F., and Seydoux, G. (2021). Regulation of biomolecular condensates by interfacial protein clusters. Science 373, 1218-1224. (co-first author). Link
5. Schmidt, H., Putnam, A., Rasoloson, D., and Seydoux, G. (2021). Protein-based condensation mechanisms drive the assembly of RNA-rich P granules. ELife 10, e63698. Link
6. Lee, C.-Y.S., Putnam, A., Lu, T., He, S., Ouyang, J.P.T., and Seydoux, G. (2020). Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins. Elife 9. Link
7. Putnam, A., Cassani, M., Smith, J., and Seydoux, G. (2019). A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat Struct Mol Biol 26, 220–226. Link

For more publications visit this link.