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Notes on Autoclave Use
Notes on Autoclave Use: Orange protective gloves have been provided for each of the BMC autoclaves on floors 4-5-6, and the three large autoclaves in B2422. All the gloves have been marked as to which autoclave they belong to. Please exercise care (and consideration) by not walking away with these protective gloves. They were meant to keep everyone from getting burned, not just you.
If you use the autoclave, you should be around to remove your items when the run is complete. Leaving items in the autoclave overnight will put the unit in a state where it may not reset the steam valves, and allow water (condensate) to collect in the steam traps, causing problems for the next user. Please be considerate, and remove your items in a timely manner.
When not in use, the doors of all autoclaves should be left open. The reason for this is that when the autoclave doors are closed, they exert pressure on the door seals, shortening their useful life. The doors of the large autoclaves in the basement should be left open about 6″ to help in keeping room B2422 from becoming a sauna. Our autoclaves are tested monthly (see Efficacy tab for details).
If you have any problems in getting the autoclave to open after a run, try turning the green power switch to “off”, wait about 15 minutes, then turn the power switch back to “on”. This will reset the autoclave. If this doesn’t work, or if you encounter any other autoclave problems, email Joe Oliva: email@example.com .
Autoclave use guidelines from UW Safety (PDF documents):
Autoclave Efficasy Testing
Autoclave Efficacy Testing: All autoclaves normally used by BMC (floors 4-5-6, three large autoclaves in room B2422, and the Teaching Lab Autoclave, room 2126 Biochemistry) undergo monthly efficacy testing per UW Health & Environmental Safety protocol. ProSpores bacteria ampules are used for the testing, and the autoclaves are tested during a 30-minute sterilization time, 250F liquids cycle. The results are posted in a log attached to each autoclave, and on the wall in B2422. Any questions on efficacy testing should be directed to Joe Oliva.
Notes on Glassware Washer Use
Notes on Glassware Washer Use: The BMC glassware washers are located in room B2422. There are four washers in this room, two operated by BMC, and two kept in storage by the Biochemistry Dept., with who we share the resources of this room. If you have any problems with the glassware washers, email Joe Oliva firstname.lastname@example.org .
The glassware washers were installed at a cost of $238,000, and the cost of the washer racks in the adjacent room were more than $60,000. That means you are using a washing system that in total costs over a quarter of a million dollars! Please treat the washers and the racks accordingly (with great care), as there is currently no funding available to replace either. Please make sure the glassware you are about to wash is free of all tape and labels. If tape or labels get sucked into the washers, they will collect in the water pumps and cause them to fail (cost of repair several thousand dollars).
When not in use, please return the washer racks (gently) to the storage racks in the adjacent wash room.
Joe checks the glassware washer status a couple of times a week to replace the detergent and neutralizer, and clean the water filters. You may occasionally see a message flashing on the control panel:
Check DOS-1: This means the detergent is running low and needs to be changed.
Check DOS-3: This means the liquid neutralizer agent needs to be changed.
Replace Course Filter: The air filters for the dryer are due for replacement.
Replace Fine Filter: The HEPA air filters for the dryer are due for replacement.
Send Joe an email if you see any of these messages email@example.com .
BMC Autoclave and Glassware Washer Manuals
Beckman Centrifuge Manuals
Beckman Optima XPN-100 Ultracentrifuge (5th Floor)
Beckman LE-80K Ultracentrifuge (4th, 6th Floor, T-Lab)
Beckman L-7 Series Ultracentrifuge (Teaching Lab)
Beckman Avanti J-26 XP Centrifuge (6th Floor)
Beckman J2-21 Centrifuge (6th Floor, T-Lab)
Beckman High-Speed and Ultra Rotor Manuals
Beckman SW 28 and SW 28.1 Swinging-Bucket Ultra Rotors
Beckman Type 45 Ti Ultra Rotor
Beckman SW 55 Ti Swinging-Bucket Ultra Rotor
Beckman SW 60 Ti Swinging-Buckent Ultra Rotor
Beckman Type 70 Ti Fixed-Angle Ultra Rotor
Beckman Type 70.1 Ti Fixed-Angle Swinging Ultra Rotor
Beckman JA-25.50 8x50ml Fixed Angle high-speed rotor
Beckman JLA-16.250 6x250ml Fixed Angle high-speed rotor
Beckman JLA-8.1 6×1 liter Fixed Angle Rotor
Other BMC Equipment Manuals and Instructions
Other BMC Equipment Manuals:
Millipore Advantage A-10 Water System
Film Processor Condensed Operating Instructions
Film Processor Full Operating Handbook
Gel Doc Camera Instructions (Microscope Suite 5211)
Bio-Rad CFX384 PCR Machine (room 4242)
Azure C600 Imaging System Manual (Room 4243)